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菌物学报  2012 

Characteristics of the β-tubulin genes from three carbendazim-tolerant Alternaria species
三种多菌灵耐药性链格孢属真菌β-tubulin基因的序列特征

Keywords: β-微管蛋白,氨基酸,耐药性,链格孢

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Abstract:

应用两对引物,从对多菌灵具有耐药性的链格孢属3个真菌中扩增了与多菌灵耐药性相关的β-微管蛋白基因,基因长度分别为1,419bp(瓜链格孢),1,426bp(茄链格孢),1,419bp(链格孢),均包含3个内含子,编码398个氨基酸。3个链格孢属真菌与其他对多菌灵敏感的植物病原真菌β-微管蛋白氨基酸具有高度的同源性。但是,3个真菌167位氨基酸均为酪氨酸,而其他对多菌灵敏感的真菌167位均为苯丙氨酸,研究结果表明链格孢属真菌对多菌灵的耐药性可能与167位酪氨酸有关。

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