A white rot fungus F17(Schizophyllum sp.) was very effective to decolorize a wide range of structurally diverse dyes. Non-woven fabric is the best immobilized carrier of the strain. Optimal preparation condition of the strain immobilization is determined by the cross-test experiments. Comparing with free cells, immobilization not only enhanced the ability to decolorize dye but also kept the stable rate of decolorization in the condition of different pH values and dye concentrations. The products decolorized by immobilized F17 were determined by UV-VIS spectra assay. The absorption in visible range disappeared,but the absorption in UV range were obviously increased. The result showed that the structures of dyes had changed greatly.