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Analysis of apigenin in Blumea balsamifera Linn DC. and its inhibitory activity against aldose reductase in rat lens

DOI: 10.4236/jacen.2012.11005, PP. 28-33

Keywords: Blumea balsamifera, Apigenin, Aldose Reductase Inhibition, HPLC

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Abstract:

To investigate the therapeutic potentials of na- tural sources, stepwise polarity fractions of Blumea balsamifera were tested for their ability to inhibit aldose reductase (AR) activity in rat lenses. Of these, the ethyl acetate (EtOAc) fraction exhibited a unique AR inhibitory activity (IC50 value, 0.11 μg/mL). Apigenin was identified from the active EtOAc fraction and exhibited high AR inhibitory activity (IC50 value, 4.03 μM). The content of apigenin was measured in B. balsamifera (0.47 mg/g) by HPLC/UV analysis. Our result suggests that B. balsamifera could be a useful natural source for the development of a novel AR inhibitory agent against diabetic complications.


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