The studies were carried out of the test of fatigue crack growth rate and relative to the microscopic morphology of fracture surface in certain high-strength steels. It was found that the results measured by both macro-and microscopic examinations are identical. The fatigue striation of the subcritical crack propagation is quantitatively related to the mean stress. In the same range of stress intensity factors the crack growth rate increases with the mean stress. The fractographic observations of the fatigue crack propagation on specimens of 18CrMn2MoBA steel reveal that the microstructural particle phase constituent with martensite and austenite can be responsible for the reduction of the fatigue crack growth rate.