To find feasible and optimal method of transplant and restoration for eelgrass Zostera marina, eelgrass were transplanted using methods of rock planting, staple, free planting, root gripping and box planting in the inshore areas of Lidao Town of Rongcheng City from October to November in 2008. The changes of growth, survival and osmotic pressure between nature and transplanting eelgrass were compared during one month after transplantation. The correlation between the growth and survival of transplanting eelgrass and environmental factors was analyzed. The results in the present study showed that the survival rate of transplanting eelgrass was as follows: rock planting (100%) > staple (86.7%) > free planting (66.7%) > root gripping (20%) > box planting (0%). The average absolute growth rate of transplanting eelgrass was as follows: rock planting (0.358 cm/d) > free planting (0.242 cm/d) > control (0.211 cm/d) > staple (0.083 cm/d) > root gripping (0.067 cm/d). The osmotic pressure of transplanting eelgrass roots was significantly higher than that of nature eelgrass roots, however, the osmotic pressure of rhizomes and leaves showed opposite changes (P<0.01). There was a remarkable correlation between growth and survival of transplanting eelgrass and environmental factors such as flow velocity, light intensity, sediment and so on. These findings have provided valuable data for developing feasible and low-cost transplant and restoration technologies for injured Z. marina biome.