Study on fluorescence spectroscopy characteristics of humic acid by inoculating microbes on municipal solid waste composting
接种外源微生物生活垃圾堆肥中的胡敏酸荧光特性

During municipal solid waste composting, a significant amount of humic substances will be generated. Different microbes were inoculated for facilitating municipal solid wastes composting with industrialized technology. At initial and final stages of composting, humic acid was extracted from compost. After further purification, fluorescence spectroscopy was used to analyze spectroscopy characteristics of humic acid. The result indicated that fluorescence emission spectra had a wide band with the maximum centered at 440?nm. The excitation and synchronous spectra exhibited a number of peaks and shoulders. At the final stage, the humification degree of humic acid increased significantly. Although the fluorescence spectra had similar shape, the relative fluorescence intensities of characteristic peaks were different due to different microbes inoculated. The results of spectroscopic analysis indicated that, during municipal solid waste composting, the degree of molecular polymerization of humic acid would increased due to microbes inoculated, with the order of MS+ZJ > ZJ > MS > CK. Comparing with the fluorescence-spectroscopy characteristics of humus substance in soil, the humic acid spectra in compost were more similar to fulvic acid in soil. It indicated that humic acid from municipal solid wastes composting had high activity.