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Marine Drugs  2011 

Quantification of Dissolved and Particulate Polyunsaturated Aldehydes in the Adriatic Sea

DOI: 10.3390/md9040500

Keywords: chemical defense, aldehydes, plankton interactions, oxylipins, heptadienal

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Abstract:

Polyunsaturated aldehydes (PUA) are supposed to play critical roles in chemically-mediated plankton interactions. Laboratory studies suggest that they act as mediators of chemical defense and chemical communication. PUA are oxylipins containing an α,β,γ,δ-unsaturated aldehyde structure element and are mainly found in diatoms. We present here a detailed surface mapping of PUA during a spring bloom of the diatom Skeletonema marinoi in the Adriatic Sea. We monitored dissolved PUA, as well as particulate PUA, which are produced by phytoplankton after cell disintegration. Our survey revealed a patchy distribution of PUA and shows that at most stations S. marinoi is the major contributor to the overall PUA. Our data also suggest that lysis of a diatom bloom can contribute significantly to the dissolved PUA concentrations and that other producers, which are smaller in cell size compared to diatoms, have to be taken into account as well if the total PUA content of marine samples is considered. The analyses of samples collected in deeper water suggests that diatom contribution to PUA decreases with depth, while smaller-sized unidentified organisms take place as dominant contributors to the PUA concentrations.

References

[1]  Wichard, T; Poulet, SA; Halsband-Lenk, C; Albaina, A; Harris, R; Liu, DY; Pohnert, G. Survey of the chemical defence potential of diatoms: Screening of fifty one species for alpha,beta,gamma,delta-unsaturated aldehydes. J Chem Ecol 2005, 31, 949–958.
[2]  Ianora, A; Miralto, A. Toxigenic effects of diatoms on grazers, phytoplankton and other microbes: A review. Ecotoxicology 2010, 19, 493–511.
[3]  Miralto, A; Barone, G; Romano, G; Poulet, SA; Ianora, A; Russo, GL; Buttino, I; Mazzarella, G; Laabir, M; Cabrini, M; Giacobbe, MG. The insidious effect of diatoms on copepod reproduction. Nature 1999, 402, 173–176.
[4]  Adolph, S; Poulet, SA; Pohnert, G. Synthesis and biological activity of alpha,beta,gamma,delta-unsaturated aldehydes from diatoms. Tetrahedron 2003, 59, 3003–3008.
[5]  Ianora, A; Miralto, A; Poulet, SA; Carotenuto, Y; Buttino, I; Romano, G; Casotti, R; Pohnert, G; Wichard, T; Colucci-D’Amato, L; Terrazzano, G; Smetacek, V. Aldehyde suppression of copepod recruitment in blooms of a ubiquitous planktonic diatom. Nature 2004, 429, 403–407.
[6]  Casotti, R; Mazza, S; Brunet, C; Vantrepotte, V; Ianora, A; Miralto, A. Growth inhibition and toxicity of the diatom aldehyde 2-trans, 4-trans-decadienal on thalassiosira weissflogii (bacillariophyceae). J Phycol 2005, 41, 7–20.
[7]  Ribalet, F; Berges, JA; Ianora, A; Casotti, R. Growth inhibition of cultured marine phytoplankton by toxic algal-derived polyunsaturated aldehydes. Aquat Toxicol 2007, 85, 219–227.
[8]  Ribalet, F; Intertaglia, L; Lebaron, P; Casotti, R. Differential effect of three polyunsaturated aldehydes on marine bacterial isolates. Aquat Toxicol 2008, 86, 249–255.
[9]  Adolph, S; Bach, S; Blondel, M; Cueff, A; Moreau, M; Pohnert, G; Poulet, SA; Wichard, T; Zuccaro, A. Cytotoxicity of diatom-derived oxylipins in organisms belonging to different phyla. J Exp Biol 2004, 207, 2935–2946.
[10]  Vardi, A; Formiggini, F; Casotti, R; De Martino, A; Ribalet, F; Miralto, A; Bowler, C. A stress surveillance system based on calcium and nitric oxide in marine diatoms. PLoS Biol 2006, 4, 411–419.
[11]  Vidoudez, C; Pohnert, G. Growth phase specific release of polyunsaturated aldehydes by the diatom skeletonema marinoi. J Plankton Res 2008, 30, 1305–1313.
[12]  Pohnert, G. Wound-activated chemical defense in unicellular planktonic algae. Angew Chem Int Edit 2000, 39, 4352–4354.
[13]  Pohnert, G. Phospholipase a(2) activity triggers the wound-activated chemical defense in the diatom thalassiosira rotula. Plant Physiol 2002, 129, 103–111.
[14]  d’Ippolito, G; Tucci, S; Cutignano, A; Romano, G; Cimino, G; Miralto, A; Fontana, A. The role of complex lipids in the synthesis of bioactive aldehydes of the marine diatom skeletonema costatum. Biochim Biophys Acta 2004, 1686, 100–107.
[15]  Barofsky, A; Pohnert, G. Biosynthesis of polyunsaturated short chain aldehydes in the diatom thalassiosira rotula. Org Lett 2007, 9, 1017–1020.
[16]  Wichard, T; Pohnert, G. Formation of halogenated medium chain hydrocarbons by a lipoxygenase/hydroperoxide halolyase-mediated transformation in planktonic microalgae. J Am Chem Soc 2006, 128, 7114–7115.
[17]  Poulet, SA; Escribano, R; Hidalgo, P; Cueff, A; Wichard, T; Aguilera, V; Vargas, CA; Pohnert, G. Collapse of calanus chilensis reproduction in a marine environment with high diatom concentration. J Exp Mar Biol Ecol 2007, 352, 187–199.
[18]  Poulet, SA; Wichard, T; Ledoux, JB; Lebreton, B; Marchetti, J; Dancie, C; Bonnet, D; Cueff, A; Morin, P; Pohnert, G. Influence of diatoms on copepod reproduction. I. Field and laboratory observations related to calanus helgolandicus egg production. Mar Ecol Prog Ser 2006, 308, 129–142.
[19]  Wichard, T; Poulet, SA; Boulesteix, AL; Ledoux, JB; Lebreton, B; Marchetti, J; Pohnert, G. Influence of diatoms on copepod reproduction. Ii. Uncorrelated effects of diatom-derived alpha,beta,gamma,delta-unsaturated aldehydes and polyunsaturated fatty acids on calanus helgolandicus in the field. Progr Oceanogr 2008, 77, 30–44.
[20]  Ribalet, F; Vidoudez, C; Cassin, D; Pohnert, G; Ianora, A; Miralto, A; Casotti, R. High plasticity in the production of diatom-derived polyunsaturated aldehydes under nutrient limitation: Physiological and ecological implications. Protist 2009, 160, 444–451.
[21]  Wichard, T; Gerecht, A; Boersma, M; Poulet, SA; Wiltshire, K; Pohnert, G. Lipid and fatty acid composition of diatoms revisited: Rapid wound-activated change of food quality parameters influences herbivorous copepod reproductive success. ChemBioChem 2007, 8, 1146–1153.
[22]  Hansen, E; Ernstsen, A; Eilertsen, HC. Isolation and characterisation of a cytotoxic polyunsaturated aldehyde from the marine phytoplankter phaeocystis pouchetii (hariot) lagerheim. Toxicology 2004, 199, 207–217.
[23]  Casotti, R. Stazione Zoologica ADohrn, Napoli, Italy. Unpublished work. 2011.
[24]  Wichard, T; Poulet, SA; Pohnert, G. Determination and quantification of alpha,beta,gamma,delta-unsaturated aldehydes as pentafluorobenzyl-oxime derivates in diatom cultures and natural phytoplankton populations: Application in marine field studies. J Chromatogr B Analyt Technol Biomed Life Sci 2005, 814, 155–161.

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