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Optimization of Culture Medium and Photosynthetic Characteristics of Platymonas subcordiformis
亚心形扁藻培养基的优化及光合特性

Keywords: uniform design,Platymonas subcordiformis,photosynthetic efficiency,culture medium
均匀设计
,亚心形扁藻,光能利用效率,培养基,亚心形扁藻,培养基,优化条件,光合特性,Platymonas,subcordiformis,Photosynthetic,Characteristics,Culture,Medium,细胞生长,光能利用效率,积累,和集,活性保持,光化学,高浓度,最优,藻细胞,体系,增殖,细胞密度,结果

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Abstract:

以均匀实验设计方法优化亚心形扁藻(Platymonas subcordiformis)培养基,采用U10*(108)均匀设计实验考察NO3--N, PO43--P, 缓冲体系(Tris-HAc)三因素对亚心形扁藻生长的影响,同时也考察了与其光合作用相关的PSII光化学活性、藻液pH、集光色素3个指标的变化. 结果表明,在NO3--N 9.5 mmol/L, PO43--P 0.34 mmol/L, Tris-HAc 6.7 mmol/L的优化条件下,亚心形扁藻生长迅速,15 d藻细胞密度增殖到(8.28±0.51)×106 mL-1. Tris-HAc体系能够维系藻细胞在最优pH条件下增殖,高浓度氮源利于PSII光化学活性保持恒定和集光色素的积累,从而提高了光能利用效率,促进藻细胞生长.

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