Arabidopsis thaliana" target="_blank">Arabidopsis thaliana')" href="#">Arabidopsis thaliana;nonhost disease resistance;oomycetes;Phytophthora sojae&prev_q=Phytophthora sojae')" href="#"> Phytophthora sojae" target="_blank">Phytophthora sojae')" href="#"> Phytophthora sojae;T-DNA insertional mutants
拟南芥;非寄主抗病性;卵菌;大豆疫霉菌;T-DNA插入突变体, Open Access Library" />

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植物学报  2010 

Nonhost Interaction of Phytophthora sojae and Arabidopsis thaliana and Genetic Analysis of a Susceptible Mutant
拟南芥与大豆疫霉菌的非寄主互作及一个感病突变体的遗传分析

Keywords: Arabidopsis thaliana" target="_blank">Arabidopsis thaliana')" href="#">Arabidopsis thaliana&searchField=keyword">Arabidopsis thaliana&prev_q=Arabidopsis thaliana')" href="#">Arabidopsis thaliana" target="_blank">Arabidopsis thaliana')" href="#">Arabidopsis thaliana,nonhost disease resistance,oomycetes, Phytophthora sojae" target="_blank">Phytophthora sojae')" href="#"> Phytophthora sojae&searchField=keyword">Phytophthora sojae&prev_q=Phytophthora sojae')" href="#"> Phytophthora sojae" target="_blank">Phytophthora sojae')" href="#"> Phytophthora sojae,T-DNA insertional mutants
拟南芥
,非寄主抗病性,卵菌,大豆疫霉菌,T-DNA插入突变体

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Abstract:

Phytophthora sojae and Arabidopsis thaliana were used in this study as a nonhost plant-oomycete interaction system to investigate the genetic basis of nonhost resistance against oomycete pathogens in plants. A collection of more than 40 000 T3 A. thaliana T-DNA mutant plants representing 12 000 independent insertion lines were screened by inoculating detached leaves with P. sojae zoospores, and the susceptible mutant was re-confirmed by pathogen inoculation and cytological characterization. A number of P. sojae-susceptible A. thaliana mutants were successfully obtained, and one of them, mutant 581-51, was shown to be stably susceptible to P. sojae infection. Water-soaked lesions formed on the detached leaves within 3 days, as did oospores and sporangia 4–5 days after inoculation with P. sojae zoospores. Cytological characterization revealed the formation of haustoria-like structures. Southern analysis showed the presence of four T-DNA insertion events in the mutant. Genetic analysis indicated that the susceptibility to infection by the nonhost pathogen P. sojae in the mutant 581-51 was likely controlled by a single recessive gene.

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