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Effects of cadmium stress on the antioxidative system in Ganoderma lucidum mycelia
镉对灵芝菌丝抗氧化系统的影响

Keywords: 灵芝,,抗氧化酶系,非蛋白巯基化合物,灵芝菌丝体,氧化系统,影响,mycelia,Ganoderma,lucidum,system,antioxidative,stress,cadmium,表达强度,同工酶谱,组成型,酶带,处理,发现,抗氧化酶,胁迫,凝胶电泳分析,聚丙烯酰胺,脂氧合酶

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Abstract:

The study on the effects of different concentration (0, 10, 50, 100, 200 and 400 micromol x L(-1)) cadmium (Cd) on the antioxidative system in Ganoderma lucidum mycelia indicated that with increasing concentration of Cd, the fresh mass and the proline, total polysaccharides, and reduced polysaccharides contents of G. lucidum mycelia decreased, but non-protein thiol (NPT) content increased. At 400 micromol x L(-1) of Cd, the NPT content increased dramatically, being 5.7 times higher than control. Within the range of test Cd concentrations, the activities of CAT, GR and POD increased first and decreased then, with the peak at 100 micromol x L(-1) of Cd, while the activities of LOX and SOD increased with increasing Cd concentration, with the maximum at 400 micromol x L(-1) of Cd. Polyacrylamide gel electrophoresis analysis revealed that 100-400 micromol x L(-1) of Cd induced two additional isozymes bands of Mn-SOD, 10-200 micromol x L(-1) of Cd increased the intensity of constitutive isozymes of CAT, POD, SOD and LOX, while 400 micromol x L(-1) of Cd decreased the intensity of isozymes of POD significantly.

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