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遗传  2005 

Hairy Root Induced by Wild-type Agrobacterium rhizogenes K599 in Soybean, Cucumber and Garden Balsam in vivo
发根农杆菌K599对大豆、黄瓜和凤仙花活体感染生根的研究

Keywords: Agrobacterium rhizogenes,soybean,cucumber,garden balsam,in vivo,hairy root induction
发根农杆菌
,大豆,黄瓜,凤仙花,活体,生根

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Abstract:

The plantlets of soybean, cucumber and garden balsam were inoculated by wild-type Agrobacterium rhizogenes K599, and hairy root was induced on inoculated sites in vivo. The frequencies of hairy root induction from wound cotyledons of soybean, cucumber and garden balsam were 100%, 65% and 91% respectively. Moreover, hairy root was induced from healthy cucumber axillary bud with frequency of 10%. PCR analysis of hairy root DNA was conducted using the primers from rolC gene. The PCR results showed that all hairy root lines contained T-DNA. The established system should be ideal for studying soybean and cucumber nematode and garden balsam breeding of flower dwarf architecture.

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