The Comparision of Denatured and non-denatured PAGE-Silver- Staining Detection of PCR Product of Microsatellites 微卫星PCR产物变性与非变性PAGE-银染检测方法的比较 The Comparision of Denatured and non-denatured PAGE–Silver- Staining Detection of PCR Product of Microsatellites
The genomic DNAs from six chicken breeds in China were amplified using two microsatellite primers. The PCR products were detected by non-denatured and denatured PAGE gels respectively, and the gels were dyed by silver. There were distinct differences between the two kinds of gel. In non-denatured gels. There were many nonspecific bands while clear purposed bands were showed in denatured gels.