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遗传  2004 

The Comparision of Denatured and non-denatured PAGE-Silver- Staining Detection of PCR Product of Microsatellites
微卫星PCR产物变性与非变性PAGE-银染检测方法的比较 The Comparision of Denatured and non-denatured PAGE–Silver- Staining Detection of PCR Product of Microsatellites

Keywords: microsatellite,PAGE,PCR,silver-staining
微卫星
,PAGE,PCR,银染

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Abstract:

The genomic DNAs from six chicken breeds in China were amplified using two microsatellite primers. The PCR products were detected by non-denatured and denatured PAGE gels respectively, and the gels were dyed by silver. There were distinct differences between the two kinds of gel. In non-denatured gels. There were many nonspecific bands while clear purposed bands were showed in denatured gels.

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