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遗传  2001 

Expression of Wild-type P53 Gene and P16 Gene in Lung Adenocarcinoma Cell Lines
野生型P53、P16基因协同对肺腺癌细胞系生长抑制作用的研究 Expression of Wild-type P53 Gene and P16 Gene in Lung Adenocarcinoma Cell Lines

Keywords: wild,type,P53,gene,P16,gene,adenovirus,Anip973,AGZY8(
野生型P53基因
,P16基因,腺病毒,高转移肺癌细胞系,Anip973,低转移肺癌细胞系AGZY83-a

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Abstract:

To investigate the suppression effect of tumor suppressor genes in lung adenocarcinoma cell lines,we transferred a pair of lung adenocarcinoma cell lines with different metastasis potential,Anip973(High metastasis potential cell line) and AGZY83-a (Low metastasis potential cell line)and this pair of cell lines transfected with P16 gene:AGZY83-a P16 and Anip973 P16 with wild type P53 gene with adenovirus vector.The suppression effects of P53 gene were evaluated by cell growth curve,MTT,western blotting analysis and TUNEL technique.Overexpression of wild type P53 gene in AGZY83-a,Anip973,Anip973 P16 and AGZY83-a P16 inhibited the growth of these four kinds of lung cancer cells and induced apoptosis of the cells.The suppression effect of P53 gene in Anip973 and Anip973 P16 was higher than AGZY83-a and AGZY83-a P16 while co expression of P53 and P16 in this pair of cell lines inhibited the cells more efficiently comparing with the expression of P53 alone.Wild type P53 gene might act as a candidate gene in lung adenocarcinoma gene therapy while co transfection of P53 and P16 genes was a more effective method.

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