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Strategies for Optimizing Pichia pastoris Expression System
巴斯德毕赤酵母表达系统优化策略

Keywords: 巴斯德毕赤酵母,,表达,,优化策略

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Abstract:

巴斯德毕赤酵母 (Pichiapastoris,Pp)表达系统是目前分子生物学领域中用于表达重组蛋白的标准工具之一。Pp的如下优点是促成此表达系统在近十几年里迅速发展和被广泛应用的重要原因 :Pp为单细胞真核生物 ,生长快 ,易于分子遗传学操作 ;Pp的醇氧化酶 1 (AlcoholOxidase 1 ,AOX 1 )基因的启动子具有强诱导性和强启动性 ,适于外源基因的高水平诱导表达 ;Pp具有强烈的好氧生长偏爱性 ,可进行细胞高密度培养 ,利于大规模工业化生产 ;Pp可高水平分泌表达外源蛋白 ,纯化方便 ;Pp具有真核细胞的翻译后…

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