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Construction and Characterization of sao Gene Knock-out Mutant of Streptococcus suis Serotype 2 Chinese Highly Virulent Strain 05ZYH33
2型猪链球菌中国强毒株05ZYH33 sao基因敲除突变株的构建及其生物学功能

Keywords: Streptococcus suis serotype 2,sao gene,Knock-out mutant,Biological characteristics
2型猪链球菌
,sao基因,敲除突变体,生物特性

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Abstract:

构建2型猪链球菌(Streptococcus suis serotype 2, S. suis 2)中国强毒株05ZYH33的sao基因敲除突变株。构建中间为壮观霉素抗性基因, 两侧为sao编码基因上下游同源序列的基因敲除载体, 同源重组筛选sao基因敲除突变株。PCR、RT-PCR、Western Blot对疑似突变株进行验证, 实验结果均证实sao基因完全被spc抗性基因替代, 成功构建了突变株05ZYH33Δsao。对野生株和突变株进行菌落溶血活性、生长特性、小鼠致病性比较, 结果表明sao基因的敲除并未使野生型菌株在以上三方面产生明显的变化。筛选获得的05ZYH33 sao基因突变株为进一步研究sao基因在05ZYH33致病过程中的作用奠定了基础。

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