Apis cerana cerana were exposed to four alternative chemicals of PFOS to test the acute toxicity of these chemicals. Toxicity-evaluation methods of Apis cerana cerana to chemicals were built. Two methods of "small breaker" and "feeding tube" were used to measure the oral toxicity. The relative merits and the effects of two methods were compared. The results suggested that 24 h-LC50 and 48 h-LC50values of C4 fabric three finishing agent to Apis cerana cerana of "small breaker" were 1435 and 284.67 mg·L-1, respectively. No deaths of bee happened in the limited test of other three chemicals. The contact toxicity and the oral toxicity throughout two methods showed that four chemicals were all low toxic. While the oral toxicity throughout "small breaker" was higher than that of "feeding tube".