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THE EFFECT OF HYPOCRELLIN B ON THE FLUORESCENCE OF BACTERIORHODOPSIN
竹红菌乙素对菌紫质荧光的影响

Keywords: Hypocrellin B,Purple membrane,Bacteriorhodopsin,Fluorescence quenching
竹红菌乙素,紫膜,菌紫质,荧光猝灭

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Abstract:

The interaction between Hypocrellin B and bacteriorhodopsin was studied using fluorescence method. The effect of Hypocrellin B onn absorption spectrum of Bacteriorhodopsin, the concentration and tempererature dependence of Hypocrellin B on the fiuorescence quenching of bacteriorhodopsin showed that Hypocrellin B was a new fluorescence quencher and the intrinsic fluorescence quenching of Bacteriorhodopsin was a dynamic process.The difference of fluorescence quenching between light-adapted and dark-adapted bacteriorhodopsin showed this quencher can be used in the study of conformational change of Biomembrane proteins.

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