This study tests the paleomagnetic and proxy-data based Mid- to Upper Pleistocene sediment deposition history of Lake El'gygytgyn by applying different approaches of luminescence dating techniques on sediment cores taken from the centre of the 175 m deep lake. For dating polymineral and quartz fine grains (4–11 μm grain size range) were extracted from nine different levels from the upper 28 m of sediment cores 5011-1A and 5011-1B. According to the independent age model, the lowest sample from 27.8–27.9 m below lake bottom level correlates to the Brunhes-Matuyama (B/M) reversal. Polymineral sub-samples were analysed by infra-red stimulated luminescence (IRSL) and post-IR IRSL measured at 290 °C (pIRIR290) using single aliquot regenerative dose (SAR) sequences. SAR protocols were further applied to measure the blue light optically stimulated luminescence (OSL) and thermally-transferred OSL (TT-OSL) of fine-grained quartz supplemented by a multiple aliquot approach. Neither low temperature IRSL measurements at 50 °C nor any OSL dating approach on quartz yielded reliable results. Deconvolution of their dose response curves revealed a pseudo-increase of the dose response curves and explains the observed underestimation. The pIRIR protocol applied to polymineral fine grains was the only luminescence technique able to provide dating results of acceptable accuracy up to ca. 700 ka when correlated to the existing proxy-data and paleomagnetic based age record. We present the potential and limits of the different dating techniques and a correlation of pIRIR290 results with the proxy-data based age model.