Seven varieties of pigeonpea (Cajanus cajan [L.] Millsp.) of varying growth durations and adapted to a wide range of environments across eastern and southern Africa were evaluated for their shoot regeneration response in tissue culture. On a standardized shoot regeneration medium, the short duration varieties (ICPV 88091 and ICPV 86012) generally responded faster and better than the medium duration (ICEAP 00554 and ICEAP 00557) and long duration (ICEAP 00020, ICEAP 00040 and ICEAP 00053) varieties. However, all the tested varieties produced healthy rooted plants in vitro that could be transferred to the greenhouse where they exhibited normal growth, flowering and viable seed set. This study established the basis for genetic engineering of African pigeonpea varieties.