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Shoot organogenesis in oleaster (Elaeagnus angustifolia L.)

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Abstract:

Efficient plant regeneration through organogenesis was achieved from callus cultures derived from leaf explants of Elaeagnus angustifolia. Calli were obtained on MS medai containing 3% sucrose and different concentrations of TDZ (0.5, 1, 2, 3, 4 and 6 mg/l). Maximum percentage response for callus formation was 85% on MS medium supplemented with 2 mg/l of TDZ. Shoot organogenesis was achieved after transfer of calli to MS media with 3% sucrose and different concentrations of BA (0.5, 1, 2, 3, 4 and 6 mg/l) and kinetin (0.5, 1, 2, 3, 4 and 6 mg/l). Maximum organogenesis was obtained with 2 mg/l of BA. Rooting of the shoots was achieved on MS supplemented with 0.2 mg/l of IBA. Regenerated plantlets were acclimatized and successfully transplanted to soil.

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