The aim of this paper was the detection of specific antibodies anty-Borrelia in the sera of 208 dogs from the area of Lublin District. ELISA tests, and Western blott were conducted. In ELISA tests 3.85% sera of dogs were positive for spirochetes. All sera that were positive in ELISA for spirochetes (8 samples) were examined additionally in Western blott method for the presence of antibodies against Borrelia afzelii. In all 8 sera, specific antibodies against Borrelia were detected. In all cases immunoglobins IgG reacted strongly with antigens: 30, 33, 44, 56, 64, 68 kDa of B. afzelii. Low seroprevalence against borreliosis in examined dogs indicates that the area of Lubelskie area endemic place of Lyme disease however, is not an veterinarians should keep in mind borreliosis in differential diagnosis of tick-borne diseases in dogs in this region.