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A novel method of DNA shuffling without PCR process

DOI: 10.1007/BF03184266

Keywords: DNA shuffling,restriction enzyme,tissue plasminogen activator

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Abstract:

Most DNA shuffling methods currently used require PCR process. A novel method of DNA shuffling without PCR process is described, taking advantage of the feature of some restriction enzymes whose recognition sites differ from their cleavage sites, thus giving rise to different cohesive ends. These cohesive ends can be rejoined at their native sites from different parental sequences, generating new sequences with various combinations of mutations.

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