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Comparación de los métodos PetrifilmTM coliformes y Número Más Probable (NMP) para la determinación de coliformes fecales en muestras de queso blanco

Keywords: fecal coliform, petrifilmtm, most probable number method, white cheese.

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Abstract:

fecal coliform belong to an important group of sanitary quality indicator microorganisms in food, mainly constituted for escherichia coli, considerated as indicators of recent fecal contamination, that is why it is very important to investigate their presence and to detect their population in food rapidly. the objetive of this study was to compare the most probable number (npm) method for fecal coliform determination, according to venezuelan standard covenin no 1104-96, with the coliform petrifilmtm 3mtm plates, incubated in circulating thermostatically - controlled water bath (pba) at 45 ± 0,2 ° c for 24 ± 2 hours and in a circulating air incubator at 44 ± 1° c for 24 ± 2 hours (pes), according to the recommendation of association francoise of normalization, paris (afnor) and 3mtm corporation. forty-two white cheese samples were analyzed using both methods mentioned above. they were dispensed at the same time with decimal dilutions of the samples. data generated were subjected to correlation of related samples (sps 7.5 version) and the correlation coefficients (r) were obtained; r = 0.952 nmp and pes; r = 0.944 nmp and pba. it is interesting to observe a good correlation between the methodologies in their different forms for fecal coliform determination in white cheese. coliform petrifilmtm plates incubated at the optimal temperature of coliform fecal culture represent a rapid alternative and reliable method for the assessment of fecal coliform population in white cheese.

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