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Avalia??o da regenera??o hepática com modula??o pelo pré-condicionamento isquêmico após isquemia e reperfus?o e hepatectomia parcial

DOI: 10.1590/S0100-69912012000300009

Keywords: hepatic regeneration, ischemic preconditioning, ischemia, reperfusion, hepatectomy.

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Abstract:

objective: to evaluate liver regeneration modulated by ischemic preconditioning after ischemia, reperfusion and partial hepatectomy. methods: we used 24 wistar rats of 12 weeks of age, which were randomly assigned into four groups: control (sham), hepatectomy (hep), ischemia and reperfusion (irg) and ischemic preconditioning (srg). analyses were made on liver enzymes alt and ast, assessment of regeneration through the initial and final weight of the liver and the proliferation of hepatocytes by immunohistochemical analysis with proliferating cell nuclear antigen (pcna). results: in all groups there was liver regeneration, with no statistically significant difference between them. there were significant differences in alt and ast between groups hep and sham, pre and gir, gir and sham and pre and sham (p <0.05). there were also significant differences in the pcna labeling of the sham group as compared to other groups (p <0.05). conclusion: the ischemic preconditioning decreased liver injury, but did not influence the regeneration up to 48 hours.

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