OALib 期刊
  OALib Journal (ISSN Print: 2333-9705, ISSN Online: 2333-9721)是一本多合一的开源期刊,以同行评审的方式出版发行文章,其所涵盖的研究领域多达311种领域。本刊发表的全部文章均可在期刊网站上免费阅读、下载、引用和传播。单篇文章出版费用为99美元。详情请咨询[email protected] 或 QQ: 3279437679。现在就去投稿!
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Feb 28, 2019Open    AccessArticle

Formation of Cell-Type-Associated Chromatin Configurations: A Hypothesis

Gao-De Li
More than 30 years ago, we published a hypothesis in which the 3-dimen- sional structure of entire chromatin fibres in a eukaryotic cell is named as chromatin configuration that determines the pattern of gene expression in each type of cell. Based on this hypothesis, cell differentiation is the process in which different types of cells are endowed with different chromatin configura-tions. Therefore, this chrom
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Feb 01, 2019Open    AccessArticle

Further Thoughts on Abnormal Chromatin Configuration and Oncogenesis

Gao-De Li
More than 30 years ago, we published a paper entitled as “Abnormal Chromatin Configuration and Oncogenesis”, which proposed the first hy-pothesis that links oncogenesis to abnormal three-dimensional (3D) genome structure. Recently, many studies have demonstrated that the 3D genome structure plays a major role in oncogenesis, which strongly supports our hypothesis. In this paper, further thoughts about our hypothesis are presented.
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Jun 26, 2018Open    AccessArticle

The Origin of Life Discovered: ΣRNA

Muying Zhou
Since 1992, scientific knowledge has been enough to determine the origin of life: “life originated through RNA”. The truth is that current life world on Earth is just an RNA world, since the protein is created by RNA, then DNA as life’s database is created by RNA along with reverse transcriptase (a protein of RNA making). The two life series on the planet originated through two RNA groups (named ΣRNA ): IΣRNA and IIΣRNA . IΣRNA
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Jan 30, 2018Open    AccessArticle

Characterization of Chitosan Membranes Crosslinked by Sulfuric Acid

Thiago B. Fideles, José Luis Santos, Helena Tomás, Glória T. F. S. Furtado, Daniel B. Lima, Silvia M. P. Borges, Marcus V. L. Fook
Development of novel biomaterials and its practical application have been the subject of much research in the field of scaffolds for tissue engineering, providing the success of producing scaffolds biomaterials that facilitate tissue growth and provide structure support for cells. Due to its biocompatibility and biodegradability, chitosan has been the focus of several researches in recent years to be applied in the
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May 05, 2017Open    AccessArticle

Electronmicroscopic Study of Nanolike Bacteria

Karlen Hovnanyan, Margarita Hovnanyan, Armen Trchounian
Nanobacteria individual cases under the name of suggesting the bacteria mycoplasma and L-transformed forms of bacteria that imitate their size are nanobacteria that a separate systematic units remains controversial; the introduction of new high-resolution analytical microscopy techniques of prokaryote and eukaryote has revealed new structures in the structures of bacteria, which are the target of physical, chemical and biologicall
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Mar 14, 2017Open    AccessArticle

Cell-Cycle-Associated Amplified Genomic-DNA Fragments (CAGFs) Might Be Involved in Chloroquine Action and Resistance in Plasmodium falciparum

Gao-De Li
As a cheap and safe antimalarial agent, chloroquine (CQ) has been used in the battle against malaria for more than half century. However, the mechanism of CQ action and resistance in Plasmodium falciparum remains elusive. Based on further analysis of our published experimental results, we propose that the mechanism of CQ action and resistance might be closely li
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Nov 07, 2016Open    AccessArticle

Is Absence of Spontaneous Agglutinates of Spermatozoa in Semen a Reliable Indicator of Non-Autosensization against Human Sperm Antigens?

Antoine Laudat, Laurence Burc-Struxiano
Objective: Here we compared the results of a prospective study systematically screening for antisperm antibodies in a cohort of subfertile males to the results of a previous retrospective study in equally subfertile patients where screening for antisperm antibodies was performed solely if semen presented spontaneous agglutinates of sperm. Methods: The prospective study was conducted on 317 semen analyses between 1 September 2014 a
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Aug 17, 2016Open    AccessArticle

Genoautotomy (Genome 'Self-Injury') in Eukaryotic Cells: A Cellular Defence Response to Genotoxic Stress

Gao-De Li
This paper proposes that eukaryotic cells, under severe genotoxic stress, can commit genoautotomy (genome “self-injury”) that involves cutting and releasing single-stranded DNA (ssDNA) fragments from double-stranded DNA and leaving ssDNA gaps in the genome. The ssDNA gaps could be easily and precisely repaired later. The released ssDNA fragments may play some role in the regulation of cell cycle progression. Taken together, genoau
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Jul 26, 2016Open    AccessArticle

Targeted Mutagenesis of Beta-Lactoglobulin Gene in Caprine Fetal Fibroblasts by Context-Dependent Assembly Zinc-Finger Nucleases

Yuguo Yuan, Yong Cheng, Jinyu Wang, Qiuling Peng
Targeted mutagenesis by zinc-finger nucleases (ZFNs) can be used to generate knock-out mammalian cell lines with high efficiency. A number of different methods have been developed for the design and assembly of gene-specific ZFNs, making them easily accessible to researchers. In this study, we used ZFNs assembled through the CoDA (context-dependent assembly) platforms to generate mutant caprine fetal fibroblasts cells for the BLG
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Jun 17, 2016Open    AccessArticle

A Possible Mechanism of DNA to DNA Transcription in Eukaryotic Cells: Endonuclease Dependent Transcript Cutout

Gao-De Li
We previously proposed the existence of DNA to DNA transcription in eukaryotic cells, but the mechanism by which single-stranded DNA (ssDNA) transcript is produced and released from the genome remains unknown. We once speculated that the mechanism of DNA to DNA transcription might be similar to that of DNA to RNA transcription, but now we propose that endonuclease dependent transcript cutout may be a possible mechanism of DNA to D
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