%0 Journal Article
%T Optimization of Fermentation Conditions for <i>¦Ã</i>-PGA Production by <i>Bacillus subtilis</i> QM3
%A Bingqi Dong
%A Qingping Hu
%J Open Access Library Journal
%V 11
%N 2
%P 1-15
%@ 2333-9721
%D 2024
%I Open Access Library
%R 10.4236/oalib.1111160
%X <em>¦Ã</em>-Polyglutamic acid (PGA) is a high molecular polymer polymerized by glutamic acid (Glu). In the <em>¦Ã</em>-PGA production pathway, the production of <em>¦Ã</em>-PGA by microbial fermentation in the laboratory has become the main way of <em>¦Ã</em>-PGA synthesis because of its convenient operation, low cost, and considerable effect. In order to find the high yield of <em>¦Ã</em>-PGA strain and increase the yield of <em>¦Ã</em>-PGA produced by microorganisms, <em>B. subtilis</em> QM3 was used as the experimental material to optimize the nutrition conditions and culture conditions of <em>¦Ã</em>-PGA production by QM3 fermentation. The results showed that the optimum medium composition and fermentation conditions for the production of <em>¦Ã</em>-PGA by <em>B. subtilis</em> QM3 fermentation were as follows: glucose 4%, yeast extract 1%, sodium glutamate 1%, MgSO<sub>4</sub><span style="text-wrap:nowrap;">£¿</span>7H<sub>2</sub>O 0.025%, K<sub>2</sub>HPO<sub>4</sub> 0.2% department NH<sub>4</sub>Cl 0.3%, initial pH 6, 250 ml flask, liquid volume 30 ml, 121<span style="text-wrap:nowrap;">£¿</span>C sterilization 20 min, inoculum 2%, shaker speed 220 rpm 42<span style="text-wrap:nowrap;">£¿</span>C shaker culture for 72 hours, at this time, the yield of <em>¦Ã</em>-PGA was the highest, reaching 124.58 g£¿L.
%K <i>B. subtilis</i> QM3
%K <i>¦Ã</i>-PGA
%K Condition Optimization
%U http://www.oalib.com/paper/6814921