%0 Journal Article
%T Effects of astragalus polysaccharide and astragaloside IV on lipopolysaccharides-induced inflammation of bovine mammary epithelial cells
%A FAN Jiaqi
%A JIA Fang
%A LI Yusi
%A ZHOU Xuezhang
%J Journal of South China Agricultural University
%R 10.7671/j.issn.1001-411X.202106034
%X ¡¾Objective¡¿ To investigate the effects of astragaloside IV (AS-IV) and astragalus polysaccharides (APS) of astragalus extract on the lipopolysaccharides (LPS)-induced inflammation of bovine mammary epithelial cells (BMECs) and the effect on the expression of ¦Â-casein in inflammatory cells. ¡¾Method¡¿ APS and AS-IV were used to intervene LPS-induced BMECs to detect the changes of cell inflammatory factors, oxidative factors, apoptosis factors and ¦Â-casein. ¡¾Result¡¿ CCK-8 screening found the concentrations of 1g/L APS and 50, 75, 100 mg/L AS-IV were optimal to stimulate BMECs. After stimulating BMECs with 0.5 mg/L LPS for 24 h, the inflammatory cell model was successfully established. Experiments found that AS-IV and APS could significantly inhibit the expression of lactate dehydrogenase (LDH) in inflammatory cells and alleviate the continuous damage of cell membranes in the inflammatory state; Significantly inhibit the expression of malondialdehyde (MDA) and reactive oxygen species (ROS) in inflammatory cells and alleviate the oxidative damage of cells; Significantly inhibit the expression of IL-6, IL-8, TNF-¦Á inflammatory factors in inflammatory cells, and reduce the inflammatory response of cells; Significantly inhibit the expression of apoptotic proteins in inflammatory cells and inhibit cell apoptosis; Significantly activate ¦Â-casein in BMECs inflammatory cells and normal cells, and inhibit the expression of TGF-¦Â and ERK1/2. ¡¾Conclusion¡¿ AS-IV and APS can inhibit the inflammatory response of BMECs induced by LPS and activate the expression of ¦Â-casein in inflammatory cells at the optimal concentrations.
%K Astragalus polysaccharide
%K Astragaloside IV
%K Inflammation
%K Bovine
%K Mastitis
%K Epithelial cell
%K ¦Â-casein
%U https://xuebao.scau.edu.cn/zr/html/2022/4/20220403.htm