%0 Journal Article
%T Microbial Inoculants Development for Bioremediation of Gasoline and Diesel Contaminated Soil
%A Aline Jaime Leal
%A Edmo Montes Rodrigues
%A Rita de C¨¢ssia Rocha Fernandes
%A Arnaldo Chaer Borges
%A Aline Daniela Lopes J¨²lio
%A Fernanda de Souza Freitas
%A Marcos Rog¨¦rio T¨®tola
%J Open Access Library Journal
%V 5
%N 4
%P 1-17
%@ 2333-9721
%D 2018
%I Open Access Library
%R 10.4236/oalib.1104449
%X
In this study, we describe the development of
microbial inoculants for the bioremediation of hydrocarbon-contaminated soils
through the enrichment of hydrocarbonoclastic populations in
municipal solid waste compost (MSWC). Respirometric analyses were performed
along with quantification of total heterotrophic bacteria and ester-linked
fatty acid methyl ester (EL-FAME) profiling of the microbial communities of the
inoculants. CO2-emission rate increased sharply when the compost
received application of water plus gasoline or diesel. After 8 (compost diesel)
and 12 days (compost gasoline), we observed a significant increase in the
number of heterotrophic bacteria. In inoculants receiving gasoline, FAME
markers of fungi predominated throughout the incubation period (18 days). By
the end of the incubation period, an increase in FAMEmarker for gram-positive
bacteria and a decrease for gram-negative bacteria and actinobacteria were
observed. In biodegradation trials (data not shown), the inoculants were very
efficient, removing over 99% of hydrocarbons from a heavy soil (73% clay)
contaminated with either diesel or gasoline (17,000
mg¡¤Kg©1 and 15,000
mg¡¤Kg©1, respectively). Inoculants based on MSWC enriched in
hydrocarbonoclastic microorganisms may be an effective alternative to improve
bioremediation in hydrocarbon-contaminated soils.
%K Bioremediation
%K Hydrocarbon Degradation
%K Inoculant Development
%K Soil Con-tamination
%U http://www.oalib.com/paper/5293251