%0 Journal Article
%T Effects of Extract and Phenol Glycoside from Rose Petals on the Amylin Fibrils
%A Karlen Hovnanyan
%A Svetlana Sharoyan
%A Alvard Antonyan
%A Narek Hovnanyan
%A Sona Mardanyan
%J Open Access Library Journal
%V 4
%N 1
%P 1-9
%@ 2333-9721
%D 2017
%I Open Access Library
%R 10.4236/oalib.1103343
%X
Peptide amyloidoses are considered as causes of a variety
of pathologies (Alzheimer¡¯s disease, Parkinson¡¯s disease, type 2 diabetes, etc.). In
the present work, the results of the transmission
and scanning electron microscopy (TEM and SEM) were used to study the
effects of the ethanol extract of rose petals and phenol glycoside fraction,
isolated from it, on the fibrillation
of the amyloid polypeptide amylin (AIAPP), which is toxic for islet cells. At TEM and SEM visualization of amylin fibrils, the size,
form-factor, distribution by dimension and by polymorphism degree were taken
into account. The nature of conformational diversity of aggregates of varying
degree was analyzed. The analyses showed simultaneous presence of
various structural forms: protofibrils, mature fibrils and ribbon-like forms.
In case of plant preparations, a) inclusions of their particles caused increase
of fibril dimention; b) amorphous bundles without clear configuration of the
structure appeared, etc. These observations are in concordance with the earlier
observed ability of these plant preparations to hinder the amylin fibrillation
and to protect the islet cells from the toxicity of aggregated amylin. The
findings of the present work demonstrate TEM and SEM as reasonable approaches
in seeking effective antiamyloidogenic agents.
%K Electron Microscopy
%K Amylin
%K Amyloidosis
%K Ethanol Extract of Rose Petals
%K Phenol Glycoside from Rose Petals
%U http://www.oalib.com/paper/5281041