%0 Journal Article
%T Designing, Optimization and Validation of Tetra Primer ARMS-PCR Protocol for Genotyping Single Nucleotide Polymorphism rs4731702 (C/T) of KLF14 Gene Associated with Type 2 Diabetes Mellitus: A Study in San Luis, Argentina
%A Micaela Fernanda Alvarez
%A Miriam Ester Vasquez Gomez
%A Susana Siewert
%J Open Access Library Journal
%V 3
%N 12
%P 1-11
%@ 2333-9721
%D 2016
%I Open Access Library
%R 10.4236/oalib.1103270
%X
New, quick, and inexpensive methods for genotyping KLF14 gene polymorphism through Tetra
Primer ARMS-PCR were developed in the present investigation. Single nucleotide
polymorphism (SNP) genotyping needs to be attempted to establish association
between the identified SNP with metabolic trait for identification of molecular
markers that can be used to identify individuals at risk of developing Diabetes
Mellitus Type 2. In the current study, we have successfully genotyped the SNP
rs4731702 (C/T) in KLF14 gene. Tetra
primer ARMS-PCR protocol was optimized and validated for this SNP with short
turn-around time and costs. The optimized techniques were tested on 60 samples
of controls and type 2 Diabetes Mellitus individuals. Samples with known
genotypes for the described gene, previously tested in duplicate using the sequencing
methods, were employed for validation of the assay. Upon validation, complete
concordance was observed between the Tetra Primer ARMS-PCR assays and the
sequencing results. These results highlight the ability of Tetra Primer
ARMS-PCR in genotyping of SNP in KLF14
gene. Our results provide direct evidence that Tetra Primer ARMS-PCR is a
rapid, reliable, and cost-effective method for SNP genotyping of KLF14 gene in type 2 Diabetes Mellitus
individuals.
%K Molecular Markers
%K SNP
%K Insulinemia
%K Metabolic Syndrome
%K Dyslipidemias
%U http://www.oalib.com/paper/5277914