%0 Journal Article
%T Morphology and Biochemistry of Microbial Antagonists and Their Effect in the Growth of Garbanzo and Control of Fusariosis
%A Luz del Carmen Oliva Ortiz
%A Teresa de Jes¨²s Vel¨¢zquez Alcaraz
%A Rogelio Sosa P¨¦rez
%A Leopoldo Partida Ruvalcaba
%A Tom¨¢s D¨ªaz Vald¨¦s
%A Julio Arciniega Ramos
%A Jacobo Enrique Cruz Ortega
%J Open Access Library Journal
%V 3
%N 12
%P 1-19
%@ 2333-9721
%D 2016
%I Open Access Library
%R 10.4236/oalib.1103226
%X <div style="text-align:justify;">
	<span style="line-height:1.5;">The chickpea (</span><i style="line-height:1.5;">Cicer</i><span style="line-height:1.5;"> </span><i style="line-height:1.5;">arietinum</i><span style="line-height:1.5;"> L.) is an important crop in the world. In this study, six
strains of indigenous rhizosphere microorganisms (T442, T3241, 7A1, 751, HRG- 050 y HRG-060) were characterized
morphologically and biochemically through catalogs and API tests, and evaluated
as growth promoters and biocontrol agents of </span><i style="line-height:1.5;">F.</i><span style="line-height:1.5;"> </span><i style="line-height:1.5;">oxysporum</i><span style="line-height:1.5;"> f. sp. </span><i style="line-height:1.5;">ciceris</i><span style="line-height:1.5;"> (</span><i style="line-height:1.5;">Foc</i><span style="line-height:1.5;">), causal agent of Fusarium wilt of chickpea under laboratory and
field conditions. Preliminary studies were performed </span><i style="line-height:1.5;">in</i><span style="line-height:1.5;"> </span><i style="line-height:1.5;">vitro</i><span style="line-height:1.5;">, evaluating antagonism
by PICR of </span><i style="line-height:1.5;">Foc</i><span style="line-height:1.5;">, in Petri dishes with PDA
culture medium; the stimulation of germination was evaluated by seed inoculation
with the same strains. In the field, chickpea seeds were inoculated with said antagonistic
strains at a concentration of 1 ¡Á 10</span><sup>8</sup><span style="line-height:1.5;"> ufc or conidia mL</span><sup>©�1</sup><span style="line-height:1.5;">, establishing eight treatments: six based on the
inoculation of seed with each antagonist strain, a chemical treatment (Benomyl)
and a witness, in block design randomized complete, evaluating the protective effect
and stimulatory in chickpea plants</span><span style="line-height:1.5;">. The strains were characterized as </span><i style="line-height:1.5;">Bacillus</i><span style="line-height:1.5;"> </span><i style="line-height:1.5;">subtilis</i><span style="line-height:1.5;">, </span><i style="line-height:1.5;">Agrobacterium</i><span style="line-height:1.5;"> </span><i style="line-height:1.5;">radiobacter</i><span style="line-height:1.5;">, </span><i style="line-height:1.5;">Pseudomonas</i><span style="line-height:1.5;"> sp., </span><i style="line-height:1.5;">Trichoderma</i><span style="line-height:1.5;"> sp., and </span><i style="line-height:1.5;">Bacillus</i><span style="line-height:1.5;"> </span><i style="line-height:1.5;">megaterium</i><span style="line-height:1.5;">, the latter with low percentage
of identification. HRG-060 (</span><i style="line-height:1.5;">Trichoderma</i><span style="line-height:1.5;"> sp.) and T442 (</span><i style="line-height:1.5;">Bacillus</i><span style="line-height:1.5;"> </span><i style="line-height:1.5;">subtilis</i><span style="line-height:1.5;">) proved to be the best biocontrol
agents in laboratory and field, as well as higher growth promoters, as significantly
reduced growth of the pathogen and the incidence of the disease in the field, and
increased the germination, greenness, biomass and yield of chickpea. The use of
strains HRG-060 y T442 to control Fusarium wilt of chickpea may be possible in the
conditions that have the central area of Sinaloa.</span> 
</div>
%K <i>Trichoderma</i>
%K <i>Bacillus</i>
%K <i>Fusarium oxysporum ciceris</i> Race 5
%K Native Strains
%U http://www.oalib.com/paper/5277053