%0 Journal Article
%T Construction and Analysis of the Immune Effects of a <em>Streptococcus agalactiae</em> Surface Protein ScpB Vaccine Encapsulated with Polylactic-Co-Glycolic Acid (PLGA)
%A Xiaoli Ke
%A Qingyong Li
%A Xiaotian Li
%A Zhigang Liu
%A Maixin Lu
%A Hong Yang
%J Open Access Library Journal
%V 3
%N 8
%P 1-11
%@ 2333-9721
%D 2016
%I Open Access Library
%R 10.4236/oalib.1102886
%X <div style="text-align:justify;">
	<span style="line-height:1.5;">In order to find an effective immune preparation to control
tilapia streptococcus disease, the </span><i style="line-height:1.5;">Streptococcus</i><span style="line-height:1.5;"> </span><i style="line-height:1.5;">agalactiae</i><span style="line-height:1.5;"> surface protein serine protease
C5a peptidase (ScpB) was cloned and the recombinant protein was encapsulated in
poly (lactide-co-glycolic acid) (PLGA) microspheres, which were comprised of biodegradable
materials. The ScpB-PLGA vaccine was then administered to the tilapia intraperitoneally
at diffe</span><span style="line-height:1.5;">rent concentrations, with PBS used as a control, and the relative percent
survival (RPS) of each group was calculated. Serum lysozyme and superoxide dismutase
(SOD) activity levels and antibody levels (OD</span><sub>450nm</sub><span style="line-height:1.5;">) were tested weekly
for the duration of the experiment. The results showed that the ScpB loading rate
in the PLGA microspheres was 2.55% and the encapsulation efficiency reached 48.76%.
The RPS ranged from 66.80% to 87.66%, with the highest RPS noted in group P1 (1
¦Ìg/g). The serum lysozyme, SOD and antibody (IgM) levels were significantly higher
in the vaccinated fish relative to the control groups (</span><i style="line-height:1.5;">P</i><span style="line-height:1.5;"> &lt; 0.01). These results showed that PLGA could serve as an effective
adjuvant for a ScpB vaccine and could provide relatively sustained immune protection.</span>
</div>
%K Tilapia
%K <i>Streptococcus agalactiae</i>
%K C5a Peptidase
%K Immunogenicity
%U http://www.oalib.com/paper/5270713