%0 Journal Article
%T Primers for Dengue Virus Strains Based on Their Sequence Variability
%A Raghavendra Krishnappa
%A Rajendran Paramasivam
%J Open Access Library Journal
%V 2
%N 3
%P 1-6
%@ 2333-9721
%D 2015
%I Open Access Library
%R 10.4236/oalib.1101104
%X Since DENV-1, 2, 3, and 4 determine the strains for dengue virus, their
gene sequence can be used as marker for diagnosis, amplifying and genotyping
subtypes in molecular screening reaction which
includes RT-PCR, real-time RT-PCR, nucleic acid sequence-based amplification,
microsphere- based duplexed immunoassay, and DNA microarrays. There are
many gene based PCR diagnostic kits available for screening and quantifying
dengue virus, which one to choose? Decisions on choosing the diagnostic kit are
debatable, mainly because of sequence variation of endemic dengue virus, which emphasizes
us to use region specific primer diagnostic kits for isolating dengue of the
prevailing country. But if diagnoistic industry focuses on the homologous
regions obtained after aligning sequences, each representing the country of
origin, we can design primers which can be used to detect dengue strains from
any country of origin. Gene based diagnostics kits should have primers that
should be covered for all entries present in the NCBI database with 100% total
coverage similarity and specifically only to which they were designed for.
%K NCBI
%K EBI
%K PCR
%K Primers
%K Dengue
%K Diagnostics
%K DENV-1
%K DENV-2
%K DENV-3
%K DENV-4
%K MSA-ClustalO
%U http://www.oalib.com/paper/3129822