%0 Journal Article
%T Comparative Study of the Haemagglutination Capabilities of Lectin Extracted from Submerged Cultures of Wild and Mutant Strains of <i>Schizophyllum commune</i><o:p></o:p>
%A O. A. Awoyinka
%A D. A. Aina
%A J. K. Oloke
%A O. N. Majolagbe
%A O. I. Akoni
%J Open Access Library Journal
%V 1
%N 7
%P 1-17
%@ 2333-9721
%D 2014
%I Open Access Library
%R 10.4236/oalib.1100993
%X <span style="font-family:Verdana;">Haemagglutination
studies on lectin from both wild (SCW) and mutant strains (SCM1, SCM2, SCM3) of </span><i><span style="font-family:Verdana;">Schizophyllum commune </span></i><span style="font-family:Verdana;">using human
blood were explored in this study after six days of submerged fermentation.
Haemagglutination assay showed that the lectin from all the strains showed
slight discrimination in their haemmaglutinating activity against human blood
group with strong exhibition of agglutination with blood group O while SCM3 and
SCW showed the highest and lowest haemaglutinating activity respectively with a
titre score from 4 - 256 HA. Absolute loss of haemagglutination activity was
shown by all the four </span><i><span style="font-family:Verdana;">S. commune</span></i><span style="font-family:Verdana;"> strains tested following exposure to
CuSO</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> and NH</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;">SO</span><sub><span style="font-family:Verdana;">4</span></sub><span style="font-family:Verdana;"> at 800 mM but optimized by
KCl, MgCl</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;"> at 100 mM. Optimal pH for maximal haemagglutination
activity was observed at 7.0 for SCW, 6.0 for SCM1 and 8.0 for SCM2 while SCM3
distinctly showed 5.5 and 8.5. Except for SCM3 the thermo stability of
haemagglutinating activity was found to improve with the duration of UV
irradiation. Inhibitory stu</span><span style="font-family:Verdana;">dy in the presence of sugar showed that the
partially purified protein from all the strains of </span><i><span style="font-family:Verdana;">S. commune</span></i><span style="font-family:Verdana;"> in this study was mannose dependent lectin.</span>
%K <i>Schizophyllum commune</i>
%K Haemagglutination
%K Lectin
%K Blood Group
%U http://www.oalib.com/paper/3125073