%0 Journal Article
%T Digestion of Mitochondrial DNA of Some Sudanese camel breeds using three different Restriction Enzymes
%A M. Sulieman
%A Yossra A. Malik
%A S. Sid Ahmed
%A A. A. Makkawi
%A Nahid Gornas
%J Open Access Library Journal
%V 1
%N 4
%P 1-7
%@ 2333-9721
%D 2014
%I Open Access Library
%R 10.4236/oalib.1100667
%X <p class="MsoNormal">
	<span lang="EN-US">The purpose of this study was to
estimate relationship between and within some Sudanese camel breeds (Kababeish,
Shanabla and Nyalawei) by digestion of mitochondrial DNA using three different
restriction enzymes. DNA was extracted from 45 blood samples of Sudanese camels
(15 form each breed). Polymerase chain reaction was done using specific primers
in order to amplify the D-Loop region. The PCR products were digested using 3
different restriction enzymes (tag1, hinf and scal). The products were run on
agarose gel 2%. The result of this study revealed differences between the three
breeds according to digested and undigested samples. Tag1 did not cut any of
the 45 samples of all breeds, while scal cut 4.4% of the Shanabla breed, 6.7%
of Kababeish breed, but did not cut any of Nyalawei camels. The third
restriction enzyme hinf cut all sample from Shanabla camels, and also cut 95.6%
of Kababeish breed and also 95.6% of Nyalawei camels.</span>
</p>
%K Mitochondrial DNA
%K Camel
%K Sudan
%U http://www.oalib.com/paper/3096222