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Identification of Petroleum Degrading Bacteria and Construction of Petroleum Degrading Agent

DOI: 10.4236/oalib.1105335, PP. 1-10

Subject Areas: Environmental Sciences, Microbiology

Keywords: Petroleum Degrading Bacteria, Petroleum Degrading Agent, Degradation Characteristics

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Abstract

The aim of this study is to construct petroleum degrading agent (PDA) which can effectively degrade oil. By enrichment, domestication, and separation of culture from the soil sample of Qianjiang Guanghua Oilfield, the pure culture of three petroleum degrading bacteria G-40, G-53, and G-94 was identified from the medium supplemented with oil, which served as the sole source of carbon. The species of G-40 and G-53 were preliminarily identified and classified by morphological observation, physiological and biochemical determination, and sequence analyses of 16S rDNA. The species of G-94 was preliminarily identified and classified by morphological observation, physiological and biochemical determination, and sequence analyses of ITS rDNA. The optimal inoculation proportion of these three bacteria strains and bran proportion in composition of PDA were determined through orthogonal test. G-40, G-53, and G-94 were isolated and identified as Brevibacillus laterosporus, Tsukamurella inchonensis, and Candida tropicalis, respectively. To construct petroleum degrading agent, the optimum inoculation proportion of the three bacteria strains was A1B3C3 (G-40:G-53:G-94 = 1:4:4); and the optimum proportion of bran was D1E1F2 (soybean meal:corn flour:bran = 1:1:2). The oil removal rate of the constructed petroleum degrading agent reached to 42.32% on day 10 under the optimal proportion of bacteria inoculation and bran composition. Petroleum degrading bacteria can effectively degrade petroleum for its own growth. This study identified three petroleum degrading bacteria strains and proposed a petroleum degrading agent by studying the optimal inoculation proportion of the three bacterial strains and the accompanying bran. Our research could provide potential microbial resources for bioremediation of petroleum-contaminated soil.

Cite this paper

Tao, X. , Zhan, Y. , Jiang, T. and Hu, C. (2019). Identification of Petroleum Degrading Bacteria and Construction of Petroleum Degrading Agent. Open Access Library Journal, 6, e5335. doi: http://dx.doi.org/10.4236/oalib.1105335.

References

[1]  Aboushanab, R.A., Eraky, M. and Haddad, A.M. (2016) Characterization of Crudeoil Degrading Bacteria Isolated from Contaminated Soils Surrounding Gas Stations. Bulletin of Environmental Contamination and Toxicology, 97, 684-688.
https://doi.org/10.1007/s00128-016-1924-2
[2]  Chan, Y., Jun, Y. and Minmin, C. (2014) Polycyclic Aromatic Hydrocarbons Degrading Microflora in a Tropical Oil-Production Well. Bulletin of Environmental Contamination and Toxicology, 93, 632-636.
https://doi.org/10.1007/s00128-014-1371-x
[3]  Cohen, J.H., Mccormick, L.R. and Burkhardt, S.M. (2014) Effects of Dispersant and Oil on Survival and Swimming Activity in a Marine Copepod. Bulletin of Environmental Contamination and Toxicology, 92, 381-387.
https://doi.org/10.1007/s00128-013-1191-4
[4]  April, T.M., Foght, J.M. and Currah, R.S. (2000) Hydrocarbon-Degrading Filamentous Fungi Isolated from Flare Pit Soils in Northern and Western Canada. Canadian Journal of Microbiology, 46, 38-49. https://doi.org/10.1139/cjm-46-1-38
[5]  Al-Sayegh, A., Al-WahaibiEmail, Y., Al-Bahry, S., Elshafie, A., Al-Bemani, A. and Joshi, S. (2015) Microbial Enhanced Heavy Crude Oil Recovery through Biodegradation Using Bacterial Isolates from an Omani Oil Field. Microbial Cell Factories, 14, 141-151. https://doi.org/10.1186/s12934-015-0330-5
[6]  Leahy, J.G. and Colwell, R.R. (1990) Microbial Degradation of Hydrocarbons in the Environment. Microbiological Reviews, 52, 305-315.
[7]  Esmaeil, A.L.S., Drobiova, H. and Obuekwe, C. (2009) Predominant Culturable Crude Oil-Degrading Bacteria in the Coast of Kuwait. International Biodeterioration & Biodegradation, 63, 400-406. https://doi.org/10.1016/j.ibiod.2008.11.004
[8]  Jurelevicius, D., Alvarez, V.M. and Peixoto, R. (2013) The Use of a Combination of alkB Primers to Better Characterize the Distribution of Alkane-Degrading Bacteria. PLoS ONE, 8, e66565.https://doi.org/10.1371/journal.pone.0066565
[9]  Guibert, L.M., Loviso, C.L. and Marcos, M.S. (2012) Alkane Biodegradation Genes from Chronically Polluted Subantarctic Coastal Sediments and Their Shifts in Response to Oil Exposure. Microbial Ecology, 64, 605-616.
https://doi.org/10.1007/s00248-012-0051-9
[10]  Jurelevicius, D., Cotta, S.R. and Peixoto, R. (2012) Distribution of Alkane-Degrading Bacterial Communities in Soils from King George Island, Maritime Antarctic. European Journal of Soil Biology, 51, 37-44.
https://doi.org/10.1016/j.ejsobi.2012.03.006
[11]  Zhan, Y.B., Ma, L.A. and Tao, X.L. (2017) Isolation and Identification of Two Strains of Petroleum Degrading Bacteria. Journal of Yangtze University, 14, 61-64.
[12]  Li, B.M., Ruan, Z.Y. and Jiang, R.B. (2007) Screen and Identification of Oil Degrading Bacteria and Community Construction. Soil and Fertilizer Sciences in China, 3, 68-72.
[13]  Zhao, B. and He, S.J. (2002) Microbiology Experiment. Science Press, Beijing.
[14]  Sawadogo, A., Otoidobiga, H.C. and Nitiema, L.W. (2014) Isolation and Characterization of Hydrocarbon-Degrading Bacteria from Wastewaters in Ouagadougou, Burkina Faso. Journal of Environmental Protection, 5, 1183-1196.
https://doi.org/10.4236/jep.2014.512115
[15]  Zhan, Y.B., Zhang, Q. and Chen, K.L. (2017) Isolation and Construction of Petroleum-Degrading Flora and Their Degrading Characteristics. Environmental Pollution and Control, 39, 860-864 868.
[16]  Liu, H., Yao, J. and Yuan, Z. (2014) Isolation and Characterization of Crude-Oil- Degrading Bacteria from Oil-Water Mixture in Dagang Oil Field, China. International Biodeterioration & Biodegradation, 87, 52-59.
https://doi.org/10.1016/j.ibiod.2013.11.005
[17]  Laorrattanasak, S., Rongsayamanont, W. and Khondee, N. (2016) Production and Application of Gordonia westfalica GY40 Biosurfactant for Remediation of Fuel Oil Spill. Water, Air, & Soil Pollution, 227, 325-337.
https://doi.org/10.1007/s11270-016-3031-8
[18]  Chu, W. and Kwan, C.Y. (2003) Remediation of Contaminated Soil by a Solvent/ Surfactant System. Chemosphere, 53, 9-15.
https://doi.org/10.1016/S0045-6535(03)00389-8
[19]  Hamme, J.D.V., Singh, A. and Ward, O.P. (2003) Recent Advances in Petroleum Microbiology. Microbiology and Molecular Biology Reviews, 67, 503-549.
https://doi.org/10.1128/MMBR.67.4.503-549.2003
[20]  Medinabellver, J.I., Mar?n, P. and Delgado, A. (2005) Evidence for in Situ Crude Oil Biodegradation after the Prestige Oil Spill. Environmental Microbiology, 7, 73-781.
[21]  Barsby, T., Kelly, M.T. and Andersen, R.J. (2002) Tupuseleiamides and Basiliskamides, New Acyldipeptides and Antifungal Polyketides Produced in Culture by a Bacillus laterosporus Isolate Obtained from a Tropical Marine Habitat. Journal of Natural Products, 65, 1447-1451. https://doi.org/10.1021/np0201321
[22]  Oliveira, E.J.D., Rabinovitch, L. and Monnerat, R.G. (2004) Molecular Characterization of Brevibacillus laterosporus and Its Potential Use in Biological Control. Applied and Environmental Microbiology, 70, 6657-6664.
https://doi.org/10.1128/AEM.70.11.6657-6664.2004
[23]  Huang, X., Tian, B. and Niu, Q. (2005) An Extracellular Protease from Brevibacillus laterosporus G4 without Parasporal Crystals Can Serve as a Pathogenic Factor in Infection of Nematodes. Research in Microbiology, 156, 719-727.
https://doi.org/10.1016/j.resmic.2005.02.006
[24]  Yoshizawa, K. (1978) Treatment of Waste-Water Discharged from Sake Brewery Using Yeast. Journal of Fermentation Technology, 56, 389-395.
[25]  Song, F.M. (2012) Application and Progress on Environmental Pollution Control Using Yeasts. Environmental Science & Technology, 35, 71-75 115.

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