全部 标题 作者
关键词 摘要


Pharmacognostical, Phytochemical and Antioxidant Evaluations of Guettarda calyptrata A. Rich.

DOI: 10.4236/oalib.1104921, PP. 1-10

Subject Areas: Plant Science, Pharmacology

Keywords: Guettarda calyptrata, Pharmacognostic Study, Phytochemical, Antioxidant Activity

Full-Text   Cite this paper   Add to My Lib

Abstract

Guettarda calyptrata A. Rich., originally from Cuba and known as “Contraguao”, has traditionally been used to counteract quickly and effectively burns produced by Comocladia dentate Jacq (Guao), vegetable species that secretes a highly caustic latex for skin and mucous membranes. A burn is a traumatic injury resulting in local and systemic injury with oxidative changes; in this sense the antioxidants play an important role. In order to offer aspects related to the quality and effectiveness of the plant, its pharmacognostic, phytochemical and antioxidant activity is presented. The morphoanatomical evaluation was carried out, physical-chemical parameters were determined for the crude drug and for the aqueous extract. The chemical profile of the extract was estimated by thin layer chromatography, ultraviolet-visible and quantification of total phenols by Folin-Ciocalteu and total flavonoids by the colorimetric method of aluminum trichloride (AlCl3). Finally, the antioxidant activity was tested by the FRAP and DPPH techniques. Through the pharmacognostic study, the quality specifications of the drug and the extract were established. The methods of analysis used for the chemical profile suggested the presence of flavonoids and phenols in general. The aqueous extract showed antioxidant properties by the two methods evaluated. The study of G. calyptrata provided pharmacognostic, phytochemical and effectiveness as an antioxidant evidence, aspects to consider in the possible use of the plant by our Natural and Traditional Medicine.

Cite this paper

Gaitén, Y. I. G. , Yaque, J. G. , Lizama, R. S. , Torres, Z. O. , González, A. F. , Jiménez, J. L. M. and Monan, M. (2018). Pharmacognostical, Phytochemical and Antioxidant Evaluations of Guettarda calyptrata A. Rich.. Open Access Library Journal, 5, e4921. doi: http://dx.doi.org/10.4236/oalib.1104921.

References

[1]  Martins, D. and Nunez, C.V. (2015) Secondary Metabolites from Rubiaceae Species. Molecules, 20, 13422-13495.
https://doi.org/10.3390/molecules200713422
[2]  Revathi, D. and Rajeswari, M. (2015) Chemical Profiling of Guettarda speciosa Linn. by GC-MS. International Journal of Emerging Technology and Advanced Engineering, 5, 114-118.
[3]  árboles de Cuba, B.J. (1988) Editorial Científico-Técnica. Ciudad de La Habana, 265-275.
[4]  Roig, J.T. (2014) Diccionario botánico de nombres vulgares cubanos. Tomo I. Cuarta edición. Editorial Científico-Técnica. La Habana, p. 295.
[5]  Schencke, C., Salvo, J., Vasconcellos, A. and del Sol, M. (2013) Estudio Comparativo de la Cicatrización en Quemaduras con Tratamiento en Base a Miel de Ulmo (Eucryphia cordifolia) y Vitamina C oral versus Hidrogel en Cobayos (Cavia porcellus). International Journal of Morphology, 31, 839-844.
https://doi.org/10.4067/S0717-95022013000300010
[6]  NRSP 309. (1992) Norma Ramal. Medicamentos de origen vegetal. Droga cruda. Métodos de ensayo, 16-29.
[7]  Miranda, M.M. and Cuéllar, A.C. (2000) Manual de prácticas de laboratorio. Farmacognosia y productos naturales. Ciudad Habana, 25-49, 74-79.
[8]  Gattuso, M.A. and Gattuso, S.J. (1999) Manual de procedimientos para el análisis de drogas en polvo. Editorial de la Universidad Nacional de Rosario Urquiza, Argentina.
[9]  Lou, Z.-C. (1980) General Control Methods for Vegetable Drugs. Comparative Study of Methods Included in Thirteen Pharmacopoeias: An Proposals on Their Internacional Unification. WHO/PHARM/80.502, 8-39.
[10]  Commission, C.P. (2015) Pharmacopoeia of the People’s Republic of China. Chinese Medical Science and Technology Press, Beijing, 337.
[11]  NRSP 312. (1992) Norma Ramal. Medicamentos de origen vegetal. Extractos fluidos y tinturas. Métodos de ensayo, 15-19.
[12]  Chlopicka, J., Pasko, P., Gorinstein, S., Jedryas, A. and Zagrodzki, P. (2012) Total Phenolic and Total Flavonoid Content, Antioxidant Activity and Sensory Evaluation of Pseudocereal Breads. LWT-Food Science and Technology, 46, 548-555. https://doi.org/10.1016/j.lwt.2011.11.009
[13]  Pourmorad, F., Hosseinimerhr, S.J. and Shahabimajd, N. (2006) Antioxidant Activity, Phenol and Flavonoid Contents of Some Selected Iranian Medicinal Plants. African Journal of Biotechnology, 5, 1142-1145.
[14]  Benzie, I.F. and Strain, J.J. (1996) The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “Antioxidant Power”. The FRAP Assay. Analytical Bio-chemistry, 239, 70-76.
https://doi.org/10.1006/abio.1996.0292
[15]  Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995) Use of Free Radical Method to Evaluate Antioxidant Activity. LWT-Food Science and Technology, 28, 25-30.
https://doi.org/10.1016/S0023-6438(95)80008-5
[16]  Abad-Garcia, B., Berrueta, L.A., Garmon-Lobato, S., Gallo, B. and Vicente, F. (2009) A General Analytical Strategy for the Characterization of Phenolic Compounds in Fruit Juices by High-Performance Liquid Chromatography with Diode Array Detection Coupled to Electrospray Ionization and Triple Quadrupole Mass Spectrometry. Journal of Chro-matography A, 1216, 5398-5415.
https://doi.org/10.1016/j.chroma.2009.05.039
[17]  Martínez, J., García, C., Durango, D. and Gil, J. (2012) Caracterización de propóleos provenientes del municipio de Caldas obtenido por dos métodos de recolección. Revista MVZ Córdoba, 17, 2861-2869.
https://doi.org/10.21897/rmvz.254
[18]  Afsar, T., Razak, S., Shabbir, M. and Rashid, K.M. (2018) Antioxidant Activity of Polyphenolic Compounds Isolated from Ethyl-Acetate Fraction of Acacia hydaspicaR. Parker. Chemistry Central Journal, 12, 5.
https://doi.org/10.1186/s13065-018-0373-x
[19]  Bokhari, J., Khan, M.R., Shabbir, M., Rashid, U., Jan, S. and Zai, J.A. (2013) Evaluation of Diverse Antioxidant Activities of Galium aparine. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 102, 24-29.
https://doi.org/10.1016/j.saa.2012.09.056
[20]  Naressi, M.A., Manholer, D.D., Ames, F.Q., Bersani-Amado, C.A., Formagio, A.S.N., Pereira, Z.V., Da Costa, W.F., Baldoqui, D.C. and Sarragiotto, M.H. (2015) Chemical Constituents, Anti-Inflammatory, and Free-Radical Scavenging Activities of Guettarda viburnoides CHAM. & SCHLTDL. (Rubiaceae). Química Nova, 38, 932-936.
[21]  Hsu, F.-L., Huang, W.-J., Wu, T.-H., Lee, M.-H., Chen, L.-C., Lu, H.-J., Hou, W.-C. and Lin, M.-H. (2012) Evaluation of Antioxidant and Free Radical Scavenging Capacities of Polyphenolics from Pods of Caesalpinia pulcherrima. International Journal of Molecular Sciences, 13, 6073-6088.
https://doi.org/10.3390/ijms13056073
[22]  Csepregi, K., Neugart, S., Schreiner, M. and Hideg, E. (2016) Comparative Evaluation of Total Antioxidant Capacities of Plant Polyphenols. Molecules, 21, 208.
https://doi.org/10.3390/molecules21020208
[23]  Sepahpour, S., Selamat, J., Abd Manap, Y.M., Khatib A and Abdull Razis, A.F. (2018) Comparative Analysis of Chemical Composition, Antioxidant Activity and Quantitative Characterization of Some Phenolic Compounds in Selected Herbs and Spices in Different Solvent Extraction Systems. Molecules, 23, 402.

Full-Text


comments powered by Disqus