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Comparison of an In-House Quantitative Real-Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non-B

DOI: 10.4236/oalib.1101402, PP. 1-7

Subject Areas: Immunology, HIV

Keywords: COBAS AmpliPrep/TaqMan, In-House PCR Assays, HIV-1 Non-B

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Abstract

Context: The in-house techniques or experimental methods are increasingly recommended for their low-cost reagents for the determination of the Viral Load (VL) in resource-limited settings. The objective of this study was to compare the determination of VL from HIV-1 non-B samples by an in-house technique with the COBAS AmpliPrep/TaqMan version 2.0. Method: In this cross-sectional study, 39 plasma samples from patients infected with HIV type 1 non-B from N’Djamena and Kinshasa were used to determine the VL using the two techniques. Results: The mean values of VL are respectively 4.68 ± 1.26 and 4.58 ± 1.33 log10 RNA copies/ml for the COBAS AmpliPrep/TaqMan assays and the in-house assays. A good correlation (Spearman Correlation) was obtained, with a coefficient (R2) of 0.9452. Conclusion: This study demonstrates that there is no significant difference between the results of VL determined by the COBAS AmpliPrep/TaqMan assays and the in-house assays used.

Cite this paper

Kamangu, E. N. , Chatte, A. , Boreux, R. , Susin, F. , Kalala, R. L. , Mvumbi, G. L. , Mol, P. D. , Hayette, M. and Vaira, D. (2015). Comparison of an In-House Quantitative Real-Time PCR and COBAS AmpliPrep/TaqMan Roche for Determination of Viral Load for HIV Type 1 Non-B. Open Access Library Journal, 2, e1402. doi: http://dx.doi.org/10.4236/oalib.1101402.

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